Anti-PHGDH antibody (264-533) (STJ112490)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PHGDH (264-533) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:100-1:500IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PHGDH |
| Gene ID | 26227 |
| Uniprot ID | SERA_HUMAN |
| Immunogen Region | 264-533 |
| Immunogen Sequence | EEPPRDRALVDHENVISCPH LGASTKEAQSRCGEEIAVQF VDMVKGKSLTGVVNAQALTS AFSPHTKPWIGLAEALGTLM RAWAGSPKGTIQVITQGTSL KNAGNCLSPAVIVGLLKEAS KQADVNLVNAKLLVKEAGLN VTTSHSPAAPGEQGFGECLL AVALAGAPYQAVGLVQGTTP VLQGLNGAVFRPEVPLRRDL PLLLFRTQTSDPAMLPTMIG LLAEAGVRLLSYQTSLVSD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2). |
Additional Info
| Function | Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate. |
| Protein Name | D-3-Phosphoglycerate Dehydrogenase3-Pgdh2-Oxoglutarate ReductaseMalate Dehydrogenase |
| Database Links | Reactome: R-HSA-977347 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-D-3-Phosphoglycerate Dehydrogenase antibodyAnti-3-Pgdh antibodyAnti-2-Oxoglutarate Reductase antibodyAnti-Malate Dehydrogenase antibodyAnti-PHGDH antibodyAnti-PGDH3 antibody |
Information sourced from Uniprot.org