• Western blot analysis of extracts of various cell lines, using Prohibitin 2 (Prohibitin 2 (PHB2) ) rabbit monoclonal antibody (STJ11102436) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 300 Mu g extracts of SKOV3 cells using 3 Mu g Prohibitin 2 (PHB2) antibody (STJ11102436). Western blot was performed from the immunoprecipitate using Prohibitin 2 (PHB2) antibody (STJ11102436) at a dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using Prohibitin 2 (Prohibitin 2 (PHB2) ) rabbit monoclonal antibody (STJ11102436) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of U-2 OS cells using Prohibitin 2 (PHB2) Rabbit monoclonal antibody (STJ11102436, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of NIH-3T3 cells using Prohibitin 2 (Prohibitin 2 (PHB2) ) rabbit monoclonal antibody (STJ11102436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of NIH/3T3 cells using Prohibitin 2 (PHB2) Rabbit monoclonal antibody (STJ11102436, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of U-2 OS cells using Prohibitin 2 (Prohibitin 2 (PHB2) ) rabbit monoclonal antibody (STJ11102436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Prohibitin 2 (PHB2) in paraffin-embedded human colon carcinoma using Prohibitin 2 (Prohibitin 2 (PHB2) ) Rabbit monoclonal antibody (STJ11102436) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300ug extracts of SKOV3 cells using 3ug Prohibitin 2 (PHB2) antibody (STJ11102436). Western blot was performed from the immunoprecipitate using Prohibitin 2 (PHB2) antibody (STJ11102436) at a dilition of 1:1000.
  • Western blot analysis of various lysates using Prohibitin 2 (Prohibitin 2 (PHB2) ) Rabbit monoclonal antibody (STJ11102436) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PHB2 antibody (100-200) [S6MR] (STJ11102436)

SKU:
STJ11102436

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-REA (100-200) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:3000-1:6000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHB2
Gene ID: 11331
Uniprot ID: PHB2_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Prohibitin 2 (Prohibitin 2 (PHB2) ) (Q99623).
Immunogen Sequence: QMVNISLRVLSRPNAQELPS MYQRLGLDYEERVLPSIVNE VLKSVVAKFNASQLITQRAQ VSLLIRRELTERAKDFSLIL DDVAITELSFSREYTAAVEA K
Post Translational Modifications Phosphorylated. Tyrosine phosphorylation is indirectly stimulated by IGFBP6.
Function Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors and sex steroid hormones in the nucleus. In the mitochondria, together with PHB, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan (Probable). The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner. Also regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration. Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity. Has a key role of mitophagy receptor involved in targeting mitochondria for autophagic degradation. Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and pro-inflammatory cytokine IL6. In the nucleus, serves as transcriptional co-regulator (Probable). Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. In the plasma membrane, is involved in IGFBP6-induced cell migration. Cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. (Microbial infection) Involved in human enterovirus 71/EV-71 infection by enhancing the autophagy mechanism during the infection.
Protein Name Prohibitin-2
B-Cell Receptor-Associated Protein Bap37
D-Prohibitin
Repressor Of Estrogen Receptor Activity
Database Links Reactome: R-HSA-8949664
Cellular Localisation Mitochondrion Inner Membrane
Cytoplasm
Nucleus
Cell Membrane
Isoform 1: Mitochondrion Inner Membrane
Isoform 2: Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Prohibitin-2 antibody
Anti-B-Cell Receptor-Associated Protein Bap37 antibody
Anti-D-Prohibitin antibody
Anti-Repressor Of Estrogen Receptor Activity antibody
Anti-PHB2 antibody
Anti-BAP antibody
Anti-REA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance