• Western blot analysis of extracts of various cell lines, using PGLS antibody (STJ110664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using PGLS Rabbit polyclonal antibody (STJ110664) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat stomach using PGLS rabbit polyclonal antibody (STJ110664) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PGLS antibody (STJ110664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PGLS antibody (1-258) (STJ110664)

SKU:
STJ110664

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PGLS (1-258) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGLS
Gene ID: 25796
Uniprot ID: 6PGL_HUMAN
Immunogen Region: 1-258
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human PGLS (NP_036220.1).
Immunogen Sequence: MAAPAPGLISVFSSSQELGA ALAQLVAQRAACCLAGARAR FALGLSGGSLVSMLARELPA AVAPAGPASLARWTLGFCDE RLVPFDHAESTYGLYRTHLL SRLPIPESQVITINPELPVE EAAEDYAKKLRQAFQGDSIP VFDLLILGVGPDGHTCSLFP DHPLLQEREKIVAPISDSPK PPPQRVTLTLPVLNAARTVI FVATGEGKAAVLKRILEDQE ENPLPAALVQPHTGKLCWF
Function Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
Protein Name 6-Phosphogluconolactonase
6pgl
Database Links Reactome: R-HSA-71336
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-6-Phosphogluconolactonase antibody
Anti-6pgl antibody
Anti-PGLS antibody

Information sourced from Uniprot.org

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