• Immunofluorescence analysis of U2OS cells using PGD Rabbit polyclonal antibody (STJ110021) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using PGD Rabbit polyclonal antibody (STJ110021) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PGD Rabbit polyclonal antibody (STJ110021) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Mouse heart using PGD antibody (STJ110021) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using PGD antibody (STJ110021) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat kidney using PGD antibody (STJ110021) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PGD antibody (STJ110021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PGD antibody (184-483) (STJ110021)

SKU:
STJ110021

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PGD (184-483) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGD
Gene ID: 5226
Uniprot ID: 6PGD_HUMAN
Immunogen Region: 184-483
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 184-483 of human PGD (NP_002622.2).
Immunogen Sequence: KMVHNGIEYGDMQLICEAYH LMKDVLGMAQDEMAQAFEDW NKTELDSFLIEITANILKFQ DTDGKHLLPKIRDSAGQKGT GKWTAISALEYGVPVTLIGE AVFARCLSSLKDERIQASKK LKGPQKFQFDGDKKSFLEDI RKALYASKIISYAQGFMLLR QAATEFGWTLNYGGIALMWR GGCIIRSVFLGKIKDAFDRN PELQNLLLDDFFKSAVENCQ DSWRRAVSTGVQAGIPMPC
Function Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
Protein Name 6-Phosphogluconate Dehydrogenase - Decarboxylating
Database Links Reactome: R-HSA-71336
Reactome: R-HSA-9818028
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-6-Phosphogluconate Dehydrogenase - Decarboxylating antibody
Anti-PGD antibody
Anti-PGDH antibody

Information sourced from Uniprot.org

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