• Immunohistochemistry analysis of PEG3 in paraffin-embedded mouse brain using PEG3 Rabbit polyclonal antibody (STJ114545) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PEG3 in paraffin-embedded rat brain using PEG3 Rabbit polyclonal antibody (STJ114545) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using PEG3 Rabbit polyclonal antibody (STJ114545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PEG3 antibody (195-400) (STJ114545)

SKU:
STJ114545

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PEG3 (195-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEG3
Gene ID: 5178
Uniprot ID: PEG3_HUMAN
Immunogen Region: 195-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 195-400 of human PEG3 (NP_006201.1).
Immunogen Sequence: LSLPVVAKTSFEMDREDDRD SRAYESRSQDAESYQNVVDL AEDRKPHNTIQDNMENYRKL LSLVQLAEDDGHSHMTQGHS SRSKRSAYPSTSRGLKTMPE AKKSTHRRGICEDESSHGVI MEKFIKDVSRSSKSGRARES SDRSQRFPRMSDDNWKDISL NKRESVIQQRVYEGNAFRGG FRFNSTLVSRKRVLERKRRY HFDTDG
Tissue Specificity Brain, glial cells, astrocytes, embryo, placenta, testis, ovary and uterus. In the placenta it is found in the layer of villous cytotrophoblast cells while in the ovary it is found in the cells of the ovarian stroma including the thecal layers around the follicles. Expression is highly repressed in glioma cell lines.
Function Induces apoptosis in cooperation with SIAH1A. Acts as a mediator between p53/TP53 and BAX in a neuronal death pathway that is activated by DNA damage. Acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. Possesses a tumor suppressing activity in glioma cells.
Protein Name Paternally-Expressed Gene 3 Protein
Zinc Finger And Scan Domain-Containing Protein 24
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Paternally-Expressed Gene 3 Protein antibody
Anti-Zinc Finger And Scan Domain-Containing Protein 24 antibody
Anti-PEG3 antibody
Anti-KIAA0287 antibody
Anti-ZSCAN24 antibody

Information sourced from Uniprot.org

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