| Host: |
Mouse |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat/Cow/Dog/Pig |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (C-Term) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Purification: |
Affinity purification |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-1:2000IF 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PDHA1 |
| Gene ID: |
5160 |
| Uniprot ID: |
ODPA_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
PDH-E1 Alpha Monoclonal Antibody detects endogenous levels of PDH-E1 Alpha protein. |
| Immunogen: |
Purified recombinant human PDH-E1 Alpha (C-terminus) protein fragments expressed in E.coli. |
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-389661Reactome: R-HSA-5362517Reactome: R-HSA-70268 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibodyAnti-Pdhe1-A Type I antibodyAnti-PDHA1 antibodyAnti-PHE1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
