• Immunofluorescence analysis of HeLa cells using PDE3A Polyclonal Antibody (STJ119906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using PDE3A Rabbit polyclonal antibody (STJ119906) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PDE3A antibody (STJ119906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PDE3A antibody (380-660) (STJ119906)

SKU:
STJ119906

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE3A (380-660) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE3A
Gene ID: 5139
Uniprot ID: PDE3A_HUMAN
Immunogen Region: 380-660
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-660 of human PDE3A (NP_000912.3).
Immunogen Sequence: LLSTQLTFQAIHKPRVNPVT SLSENYTCSDSEESSEKDKL AIPKRLRRSLPPGLLRRVSS TWTTTTSATGLPTLEPAPVR RDRSTSIKLQEAPSSSPDSW NNPVMMTLTKSRSFTSSYAI SAANHVKAKKQSRPGALAKI SPLSSPCSSPLQGTPASSLV SKISAVQFPESADTTAKQSL GSHRALTYTQSAPDLSPQIL TPPVICSSCGRPYSQGNPAD EPLERSGVATRTPSRTDDT
Function Cyclic nucleotide phosphodiesterase with specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has also activity toward cUMP. Independently of its catalytic activity it is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling.
Protein Name Cgmp-Inhibited 3' -5'-Cyclic Phosphodiesterase 3a
Cyclic Gmp-Inhibited Phosphodiesterase A
Cgi-Pde A
Cgmp-Inhibited Camp Phosphodiesterase
Cgi-Pde
Database Links Reactome: R-HSA-418555
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Cytoplasm
Cytosol
Alternative Antibody Names Anti-Cgmp-Inhibited 3' -5'-Cyclic Phosphodiesterase 3a antibody
Anti-Cyclic Gmp-Inhibited Phosphodiesterase A antibody
Anti-Cgi-Pde A antibody
Anti-Cgmp-Inhibited Camp Phosphodiesterase antibody
Anti-Cgi-Pde antibody
Anti-PDE3A antibody

Information sourced from Uniprot.org

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