• Immunohistochemistry analysis of PDCD4 in paraffin-embedded human placenta using [KO Validated] PDCD4 Rabbit polyclonal antibody (STJ24925) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PDCD4 in paraffin-embedded human thyroid cancer using [KO Validated] PDCD4 Rabbit polyclonal antibody (STJ24925) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and PDCD4 knockout (KO) 293T cells, using [KO Validated] PDCD4 Rabbit polyclonal antibody (STJ24925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using [KO Validated] PDCD4 Rabbit polyclonal antibody (STJ24925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PDCD4 antibody (1-260) (STJ24925)

SKU:
STJ24925

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDCD4 (1-260) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDCD4
Gene ID: 27250
Uniprot ID: PDCD4_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
Immunogen Sequence: MDVENEQILNVNPADPDNLS DSLFSGDEENAGTEEIKNEI NGNWISASSINEARINAKAK RRLRKNSSRDSGRGDSVSDS GSDALRSGLTVPTSPKGRLL DRRSRSGKGRGLPKKGGAGG KGVWGTPGQVYDVEEVDVKD PNYDDDQENCVYETVVLPLD ERAFEKTLTPIIQEYFEHGD TNEVAEMLRDLNLGEMKSGV PVLAVSLALEGKASHREMTS KLLSDLCGTVMSTTDVEKS
Tissue Specificity Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Post Translational Modifications Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens.phosphorylation promotes proteasomal degradation of PDCD4.
Function Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Protein Name Programmed Cell Death Protein 4
Neoplastic Transformation Inhibitor Protein
Nuclear Antigen H731-Like
Protein 197/15a
Database Links Reactome: R-HSA-8950505
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And Cytoplasm
Predominantly Nuclear Under Normal Growth Conditions
And When Phosphorylated At Ser-457
Alternative Antibody Names Anti-Programmed Cell Death Protein 4 antibody
Anti-Neoplastic Transformation Inhibitor Protein antibody
Anti-Nuclear Antigen H731-Like antibody
Anti-Protein 197/15a antibody
Anti-PDCD4 antibody
Anti-H731 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance