• Immunohistochemistry analysis of paraffin-embedded human liver using PDCD2L antibody (STJ29531) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using PDCD2L antibody (STJ29531) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PDCD2L antibody (STJ29531) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PDCD2L antibody (1-358) (STJ29531)

SKU:
STJ29531

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDCD2L (1-358) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDCD2L
Gene ID: 84306
Uniprot ID: PDD2L_HUMAN
Immunogen Region: 1-358
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-358 of human PDCD2L (NP_115722.1).
Immunogen Sequence: MAAVLKPVLLGLRDAPVHGS PTGPGAWTASKLGGIPDALP TVAAPRPVCQRCGQPLALVV QVYCPLEGSPFHRLLHVFAC ACPGCSTGGARSWKVFRSQC LQVPEREAQDAQKQGNSLAA EDWCEGADDWGSDTEEGPSP QFTLDFGNDASSAKDVDWTA RLQDLRLQDAVLGAAHPVPP GLPLFLPYYICVADEDDYRD FVNLDHAHSLLRDYQQREGI AMDQLLSQSLPNDGDEKYE
Tissue Specificity Higher expression in lung, colon, mammary gland, cervix, stomach and small intestine.
Function Over-expression suppresses AP1, CREB, NFAT, and NF-kB transcriptional activation, and delays cell cycle progression at S phase.
Protein Name Programmed Cell Death Protein 2-Like
Alternative Antibody Names Anti-Programmed Cell Death Protein 2-Like antibody
Anti-PDCD2L antibody

Information sourced from Uniprot.org

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