• Western blot analysis of extracts of various cell lines, using PDC antibody (STJ110745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded mouse retina using PDC antibody (STJ110745) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat retina using PDC antibody (STJ110745) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat retina using PDC antibody (STJ110745) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse retina using PDC antibody (STJ110745) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PDC antibody (STJ110745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDC antibody (1-246) (STJ110745)

SKU:
STJ110745

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDC (1-246) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDC
Gene ID: 5132
Uniprot ID: PHOS_HUMAN
Immunogen Region: 1-246
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human PDC (NP_002588.3).
Immunogen Sequence: MEEAKSQSLEEDFEGQATHT GPKGVINDWRKFKLESQDSD SIPPSKKEILRQMSSPQSRN GKDSKERVSRKMSIQEYELI HKEKEDENCLRKYRRQCMQD MHQKLSFGPRYGFVYELETG KQFLETIEKELKITTIVVHI YEDGIKGCDALNSSLTCLAA EYPIVKFCKIKASNTGAGDR FSLDVLPTLLIYKGGELISN FISVAEQFAEEFFAGDVESF LNEYGLLPEREVHVLEHTK
Post Translational Modifications Light-induced changes in cyclic nucleotide levels modulate the phosphorylation of this protein by cAMP kinase.
Function May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX.
Protein Name Phosducin
Phd
33 Kda Phototransducing Protein
Protein Meka
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Cell Projection
Cilium
Photoreceptor Outer Segment
Photoreceptor Inner Segment
Isoform 2: Nucleus
Alternative Antibody Names Anti-Phosducin antibody
Anti-Phd antibody
Anti-33 Kda Phototransducing Protein antibody
Anti-Protein Meka antibody
Anti-PDC antibody

Information sourced from Uniprot.org

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