![Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded mouse kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177037/368254/STJ11104393_4__85533.1713133754.jpg?c=1 "Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded mouse kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded rat kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177037/368255/STJ11104393_5__50758.1713133755.jpg?c=1 "Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded rat kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and PEPCK/PCK2 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177037/368256/STJ11104393_6__16377.1713133757.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PEPCK/PCK2 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western blot analysis of various lysates, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177037/368257/STJ11104393_7__70899.1713133757.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
Anti-PCK2 antibody (1-280) (STJ11104393)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Phosphoenolpyruvate Carboxykinase Gtp-Mitochondrial (1-280) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PCK2 |
| Gene ID | 5106 |
| Uniprot ID | PCKGM_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MAALYRPGLRLNWHGLSPLG WPSCRSIQTLRVLSGDLGQL PTGIRDFVEHSARLCQPEGI HICDGTEAENTATLTLLEQQ GLIRKLPKYNNCWLARTDPK DVARVESKTVIVTPSQRDTV QLPPGGARGQLGNWMSPADF QRAVDERFPGCMQGRTMYVL PFSMGPVGSPLSRIGVQLTD SAYVVASMRIMTRLGTPVLQ ALGDGDFVKCLHSVGQPLTG QGEPVSQWPCNPEKTLIGH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis. |
| Protein Name | Phosphoenolpyruvate Carboxykinase Gtp - MitochondrialPepck-MPhosphoenolpyruvate Carboxykinase 2 - MitochondrialMtpck2 |
| Database Links | Reactome: R-HSA-70263 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial antibodyAnti-Pepck-M antibodyAnti-Phosphoenolpyruvate Carboxykinase 2 - Mitochondrial antibodyAnti-Mtpck2 antibodyAnti-PCK2 antibodyAnti-PEPCK2 antibody |
Information sourced from Uniprot.org