| Host: |
Rabbit |
| Applications: |
WB/IHC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Mrna (1-200) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PCIF1 |
| Gene ID: |
63935 |
| Uniprot ID: |
CAPAM_HUMAN |
| Immunogen Region: |
1-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PCIF1 (NP_071387.1). |
| Immunogen Sequence: |
MANENHGSPREEASLLSHSP GTSNQSQPCSPKPIRLVQDL PEELVHAGWEKCWSRRENRP YYFNRFTNQSLWEMPVLGQH DVISDPLGLNATPLPQDSSL VETPPAENKPRKRQLSEEQP SGNGVKKPKIEIPVTPTGQS VPSSPSIPGTPTLKMWGTSP EDKQQAALLRPTEVYWDLDI QTNAVIKHRGPSEVLPPHPE |
| Tissue Specificity | Ubiquitous. |
| Function | Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally. |
| Protein Name | Mrna2'-O-Methyladenosine-N(6--MethyltransferaseCap-Specific Adenosine MethyltransferaseCapamHcapamPhosphorylated Ctd-Interacting Factor 1Hpcif1Protein Phosphatase 1 Regulatory Subunit 121 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mrna antibodyAnti-2'-O-Methyladenosine-N(6--Methyltransferase antibodyAnti-Cap-Specific Adenosine Methyltransferase antibodyAnti-Capam antibodyAnti-Hcapam antibodyAnti-Phosphorylated Ctd-Interacting Factor 1 antibodyAnti-Hpcif1 antibodyAnti-Protein Phosphatase 1 Regulatory Subunit 121 antibodyAnti-PCIF1 antibodyAnti-C20orf67 antibodyAnti-CAPAM antibodyAnti-PPP1R121 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TDP2 knockout (KO) HeLa cells, using [KO Validated] TDP2 Rabbit polyclonal antibody (STJ119990) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98775/382823/STJ119990_1__79288.1713151323.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TDP2 knockout (KO) HeLa cells, using [KO Validated] TDP2 Rabbit polyclonal antibody (STJ119990) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
