| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PCCA (449-728) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:3000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PCCA |
| Gene ID: |
5095 |
| Uniprot ID: |
PCCA_HUMAN |
| Immunogen Region: |
449-728 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 449-728 of human PCCA (NP_000273.2). |
| Immunogen Sequence: |
ISKLITYGSDRTEALKRMAD ALDNYVIRGVTHNIALLREV IINSRFVKGDISTKFLSDVY PDGFKGHMLTKSEKNQLLAI ASSLFVAFQLRAQHFQENSR MPVIKPDIANWELSVKLHDK VHTVVASNNGSVFSVEVDGS KLNVTSTWNLASPLLSVSVD GTQRTVQCLSREAGGNMSIQ FLGTVYKVNILTRLAAELNK FMLEKVTEDTSSVLRSPMPG VVVAVSVKPGDAVAEGQEI |
| Post Translational Modifications | Acetylated. The biotin cofactor is covalently attached to the C-terminal biotinyl-binding domain and is required for the catalytic activity. Biotinylation is catalyzed by HLCS. |
| Function | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA. |
| Protein Name | Propionyl-Coa Carboxylase Alpha Chain - MitochondrialPccase Subunit AlphaPropanoyl-Coa -Carbon Dioxide Ligase Subunit Alpha |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599Reactome: R-HSA-71032 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Propionyl-Coa Carboxylase Alpha Chain - Mitochondrial antibodyAnti-Pccase Subunit Alpha antibodyAnti-Propanoyl-Coa -Carbon Dioxide Ligase Subunit Alpha antibodyAnti-PCCA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89714/358196/STJ11100778_1__20770.1713122514.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")