• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Pyruvate carboxylase (Pyruvate carboxylase (PC) ) antibody (STJ28223). Western blot was performed from the immunoprecipitate using Pyruvate carboxylase (Pyruvate carboxylase (PC) ) antibody (STJ28223) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using Pyruvate carboxylase (PC) Rabbit polyclonal antibody (STJ28223). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using Pyruvate carboxylase (PC) Rabbit polyclonal antibody (STJ28223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PC antibody (879-1178) (STJ28223)

SKU:
STJ28223

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PC (879-1178) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:10-1:100
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PC
Gene ID: 5091
Uniprot ID: PYC_HUMAN
Immunogen Region: 879-1178
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC) ) (NP_001035806.1).
Immunogen Sequence: HSMGLGSKFKEVKKAYVEAN QMLGDLIKVTPSSKIVGDLA QFMVQNGLSRAEAEAQAEEL SFPRSVVEFLQGYIGVPHGG FPEPFRSKVLKDLPRVEGRP GASLPPLDLQALEKELVDRH GEEVTPEDVLSAAMYPDVFA HFKDFTATFGPLDSLNTRLF LQGPKIAEEFEVELERGKTL HIKALAVSDLNRAGQRQVFF ELNGQLRSILVKDTQAMKEM HFHPKALKDVKGQIGAPMP
Post Translational Modifications Acetylation of Lys-748 might play a role in catalytic activity regulation.
Function Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Protein Name Pyruvate Carboxylase - Mitochondrial
Pyruvic Carboxylase
Pcb
Database Links Reactome: R-HSA-196780
Reactome: R-HSA-3371599
Reactome: R-HSA-70263
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Carboxylase - Mitochondrial antibody
Anti-Pyruvic Carboxylase antibody
Anti-Pcb antibody
Anti-PC antibody

Information sourced from Uniprot.org

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