Anti-PC antibody (879-1178) (STJ28223)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PC (879-1178) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:10-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PC |
| Gene ID | 5091 |
| Uniprot ID | PYC_HUMAN |
| Immunogen Region | 879-1178 |
| Immunogen Sequence | HSMGLGSKFKEVKKAYVEAN QMLGDLIKVTPSSKIVGDLA QFMVQNGLSRAEAEAQAEEL SFPRSVVEFLQGYIGVPHGG FPEPFRSKVLKDLPRVEGRP GASLPPLDLQALEKELVDRH GEEVTPEDVLSAAMYPDVFA HFKDFTATFGPLDSLNTRLF LQGPKIAEEFEVELERGKTL HIKALAVSDLNRAGQRQVFF ELNGQLRSILVKDTQAMKEM HFHPKALKDVKGQIGAPMP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC) ) (NP_001035806.1). |
Additional Info
| Post Translational Modifications | Acetylation of Lys-748 might play a role in catalytic activity regulation. |
| Function | Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. |
| Protein Name | Pyruvate Carboxylase - MitochondrialPyruvic CarboxylasePcb |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599Reactome: R-HSA-70263Reactome: R-HSA-70268 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Carboxylase - Mitochondrial antibodyAnti-Pyruvic Carboxylase antibodyAnti-Pcb antibodyAnti-PC antibody |
Information sourced from Uniprot.org