• Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit polyclonal antibody (STJ11105908) at 1:900 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from A-431 cells using Pan Phospho-T Rabbit polyclonal antibody (STJ11105908) at 1:900 dilution. A-431 cells were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Pan-Phospho-T antibody (STJ11105908)

SKU:
STJ11105908

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pan-Phospho-T is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance