• Western blot analysis of extracts of various cell lines, using PAM16 antibody (STJ111917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using PAM16 antibody (STJ111917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PAM16 antibody (21-125) (STJ111917)

SKU:
STJ111917

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAM16 (21-125) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAM16
Gene ID: 51025
Uniprot ID: TIM16_HUMAN
Immunogen Region: 21-125
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-125 of human PAM16 (NP_057153.8).
Immunogen Sequence: ARALRQEFAASRAAADARGR AGHRSAAASNLSGLSLQEAQ QILNVSKLSPEEVQKNYEHL FKVNDKSVGGSFYLQSKVVR AKERLDEELKIQAQEDREKG QMPHT
Tissue Specificity Ubiquitously expressed.
Function Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim16
Mitochondria-Associated Granulocyte Macrophage Csf-Signaling Molecule
Presequence Translocated-Associated Motor Subunit Pam16
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim16 antibody
Anti-Mitochondria-Associated Granulocyte Macrophage Csf-Signaling Molecule antibody
Anti-Presequence Translocated-Associated Motor Subunit Pam16 antibody
Anti-PAM16 antibody
Anti-MAGMAS antibody
Anti-TIM16 antibody
Anti-TIMM16 antibody
Anti-CGI-136 antibody

Information sourced from Uniprot.org

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