• Immunofluorescence analysis of U2OS cells using PABPC4 antibody (STJ27744) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using PABPC4 antibody (STJ27744) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using PABPC4 antibody (STJ27744) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PABPC4 antibody (STJ27744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PABPC4 antibody (350-450) (STJ27744)

SKU:
STJ27744

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PABPC4 (350-450) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PABPC4
Gene ID: 8761
Uniprot ID: PABP4_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).
Immunogen Sequence: VTEMNGRIVGSKPLYVALAQ RKEERKAHLTNQYMQRVAGM RALPANAILNQFQPAAGGYF VPAVPQAQGRPPYYTPNQLA QMRPNPRWQQGGRPQGFQGM P
Tissue Specificity Expressed at low levels in resting normal T cells.following T-cell activation, however, mRNA levels are rapidly up-regulated.
Post Translational Modifications Arg-518 is dimethylated, probably to asymmetric dimethylarginine.
Function Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.
Protein Name Polyadenylate-Binding Protein 4
Pabp-4
Poly(A-Binding Protein 4
Activated-Platelet Protein 1
App-1
Inducible Poly(A-Binding Protein
Ipabp
Cellular Localisation Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Polyadenylate-Binding Protein 4 antibody
Anti-Pabp-4 antibody
Anti-Poly(A-Binding Protein 4 antibody
Anti-Activated-Platelet Protein 1 antibody
Anti-App-1 antibody
Anti-Inducible Poly(A-Binding Protein antibody
Anti-Ipabp antibody
Anti-PABPC4 antibody
Anti-APP1 antibody
Anti-PABP4 antibody

Information sourced from Uniprot.org

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