• STJ70465 (0. 5µg/ml) staining of lysates of cell lines Daudi (A) and Caco-2 (B) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-P114-RHO-GEF/ARHGEF18 antibody (C-Term) (STJ70465)

SKU:
STJ70465-100

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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-P114-RHO-GEF/ARHGEF18 (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ARHGEF18
Gene ID: 23370
Uniprot ID: ARHGI_HUMAN
Immunogen Region: C-Term
Accession Number: NP_056133.2; NP_001124427.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
Immunogen Sequence: SAKEDASKEDVIFF
Function Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells.
Protein Name Rho Guanine Nucleotide Exchange Factor 18
114 Kda Rho-Specific Guanine Nucleotide Exchange Factor
P114-Rho-Gef
P114rhogef
Septin-Associated Rhogef
Sa-Rhogef
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-2173791
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013149
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Membrane
Apical Cell Membrane
In Unactivated Eosinophils
Distributed Around The Cell Periphery In The Perimembranous Region
In Activated Eosinophils
Relocates To The Tip Of The Nucleopod
A Membrane Structure Formed During Activation When The Nucleus Moves To One End Of The Cell
And Is Also Concentrated In Membrane Protrusions At The Opposite End Of The Cell
Localizes To The Apical Cell Membrane In Epithelial Cells
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 18 antibody
Anti-114 Kda Rho-Specific Guanine Nucleotide Exchange Factor antibody
Anti-P114-Rho-Gef antibody
Anti-P114rhogef antibody
Anti-Septin-Associated Rhogef antibody
Anti-Sa-Rhogef antibody
Anti-ARHGEF18 antibody
Anti-KIAA0521 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance