• Western blot analysis of extracts of various cell lines, using OTUD4 antibody (STJ117423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of NIH/3T3 cells using OTUD4 rabbit polyclonal antibody (STJ117423) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using OTUD4 rabbit polyclonal antibody (STJ117423) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-OTUD4 antibody (200-500) (STJ117423)

SKU:
STJ117423

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OTUD4 (200-500) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OTUD4
Gene ID: 54726
Uniprot ID: OTUD4_HUMAN
Immunogen Region: 200-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human OTUD4 (NP_001096123.1).
Immunogen Sequence: LKSKQAQQKRDYSIAAGLQY EVGDKCQVRLDHNGKFLNAD VQGIHSENGPVLVEELGKKH TSKNLKAPPPESWNTVSGKK MKKPSTSGQNFHSDVDYRGP KNPSKPIKAPSALPPRLQHP SGVRQHAFSSHSSGSQSQKF SSEHKNLSRTPSQIIRKPDR ERVEDFDHTSRESNYFGLSP EERREKQAIEESRLLYEIQN RDEQAFPALSSSSVNQSASQ SSNPCVQRKSSHVGDRKGS
Post Translational Modifications Phosphorylated on Ser-202 and Ser-204 likely by CSNK2A1-CSNK2A2 serine/threonine-protein kinase complex. Activates 'Lys-63'-specific deubiquitinase activity.
Function Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein. May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators. Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions.
Protein Name Otu Domain-Containing Protein 4
Hiv-1-Induced Protein Hin-1
Cellular Localisation Cytoplasm
Nucleus
Primarily Cytoplasmic
Alternative Antibody Names Anti-Otu Domain-Containing Protein 4 antibody
Anti-Hiv-1-Induced Protein Hin-1 antibody
Anti-OTUD4 antibody
Anti-HIN-1 antibody
Anti-KIAA1046 antibody

Information sourced from Uniprot.org

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