Anti-OTUD4 antibody (200-500) (STJ117423)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal OTUD4 (200-500) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OTUD4 |
| Gene ID | 54726 |
| Uniprot ID | OTUD4_HUMAN |
| Immunogen Region | 200-500 |
| Immunogen Sequence | LKSKQAQQKRDYSIAAGLQY EVGDKCQVRLDHNGKFLNAD VQGIHSENGPVLVEELGKKH TSKNLKAPPPESWNTVSGKK MKKPSTSGQNFHSDVDYRGP KNPSKPIKAPSALPPRLQHP SGVRQHAFSSHSSGSQSQKF SSEHKNLSRTPSQIIRKPDR ERVEDFDHTSRESNYFGLSP EERREKQAIEESRLLYEIQN RDEQAFPALSSSSVNQSASQ SSNPCVQRKSSHVGDRKGS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human OTUD4 (NP_001096123.1). |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser-202 and Ser-204 likely by CSNK2A1-CSNK2A2 serine/threonine-protein kinase complex. Activates 'Lys-63'-specific deubiquitinase activity. |
| Function | Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein. May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators. Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions. |
| Protein Name | Otu Domain-Containing Protein 4Hiv-1-Induced Protein Hin-1 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusPrimarily Cytoplasmic |
| Alternative Antibody Names | Anti-Otu Domain-Containing Protein 4 antibodyAnti-Hiv-1-Induced Protein Hin-1 antibodyAnti-OTUD4 antibodyAnti-HIN-1 antibodyAnti-KIAA1046 antibody |
Information sourced from Uniprot.org