Western blot analysis of various lysates using OSGEPL1 Rabbit pAb (STJ110328) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-OSGEPL1 antibody (1-270) (STJ110328)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110328
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General Information

Short DescriptionRabbit polyclonal OSGEPL1 (1-270) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolOSGEPL1
Gene ID64172
Uniprot IDOSGL1_HUMAN
Immunogen Region1-270
Immunogen SequenceMLILTKTAGVFFKPSKRKVY EFLRSFNFHPGTLFLHKIVL GIETSCDDTAAAVVDETGNV LGEAIHSQTEVHLKTGGIVP PAAQQLHRENIQRIVQEALS ASGVSPSDLSAIATTIKPGL ALSLGVGLSFSLQLVGQLKK PFIPIHHMEAHALTIRLTNK VEFPFLVLLISGGHCLLALV QGVSDFLLLGKSLDIAPGDM LDKVARRLSLIKHPECSTMS GGKAIEHLAKQGNRFHFDI
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human OSGEPL1 (NP_071748.2).

Additional Info

Tissue Specificity Widely expressed, with maximum expression in pituitary gland, prostate, rectum and uterus.
Function Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.
Protein Name Trna N6-Adenosine Threonylcarbamoyltransferase - Mitochondrial
N6-L-Threonylcarbamoyladenine Synthase
T(6a Synthase
O-Sialoglycoprotein Endopeptidase-Like Protein 1
Osgep-Like Protein 1
T(6a37 Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1
Trna Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1
Database Links
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Trna N6-Adenosine Threonylcarbamoyltransferase - Mitochondrial antibody
Anti-N6-L-Threonylcarbamoyladenine Synthase antibody
Anti-T(6a Synthase antibody
Anti-O-Sialoglycoprotein Endopeptidase-Like Protein 1 antibody
Anti-Osgep-Like Protein 1 antibody
Anti-T(6a37 Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1 antibody
Anti-Trna Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1 antibody
Anti-OSGEPL1 antibody
Anti-GCP1 antibody

Information sourced from Uniprot.org

Citations

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