• Western blot analysis of extracts of various cell lines, using OSGEPL1 antibody (STJ110328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using OSGEPL1 antibody (STJ110328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver damage using OSGEPL1 antibody (STJ110328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using OSGEPL1 antibody (STJ110328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using OSGEPL1 antibody (STJ110328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using OSGEPL1 antibody (STJ110328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse stomach using OSGEPL1 antibody (STJ110328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using OSGEPL1 antibody (STJ110328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-OSGEPL1 antibody (1-270) (STJ110328)

SKU:
STJ110328

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OSGEPL1 (1-270) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OSGEPL1
Gene ID: 64172
Uniprot ID: OSGL1_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human OSGEPL1 (NP_071748.2).
Immunogen Sequence: MLILTKTAGVFFKPSKRKVY EFLRSFNFHPGTLFLHKIVL GIETSCDDTAAAVVDETGNV LGEAIHSQTEVHLKTGGIVP PAAQQLHRENIQRIVQEALS ASGVSPSDLSAIATTIKPGL ALSLGVGLSFSLQLVGQLKK PFIPIHHMEAHALTIRLTNK VEFPFLVLLISGGHCLLALV QGVSDFLLLGKSLDIAPGDM LDKVARRLSLIKHPECSTMS GGKAIEHLAKQGNRFHFDI
Tissue Specificity Widely expressed, with maximum expression in pituitary gland, prostate, rectum and uterus.
Function Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.
Protein Name Trna N6-Adenosine Threonylcarbamoyltransferase - Mitochondrial
N6-L-Threonylcarbamoyladenine Synthase
T(6a Synthase
O-Sialoglycoprotein Endopeptidase-Like Protein 1
Osgep-Like Protein 1
T(6a37 Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1
Trna Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Trna N6-Adenosine Threonylcarbamoyltransferase - Mitochondrial antibody
Anti-N6-L-Threonylcarbamoyladenine Synthase antibody
Anti-T(6a Synthase antibody
Anti-O-Sialoglycoprotein Endopeptidase-Like Protein 1 antibody
Anti-Osgep-Like Protein 1 antibody
Anti-T(6a37 Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1 antibody
Anti-Trna Threonylcarbamoyladenosine Biosynthesis Protein Osgepl1 antibody
Anti-OSGEPL1 antibody
Anti-GCP1 antibody

Information sourced from Uniprot.org

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