• Immunofluorescence analysis of U2OS cells using OGFR Rabbit polyclonal antibody (STJ29154) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using OGFR antibody (STJ29154) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using OGFR antibody (STJ29154) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using OGFR antibody (STJ29154) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using (STJ29154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-OGFR antibody (1-676) (STJ29154)

SKU:
STJ29154

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OGFR (1-676) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OGFR
Gene ID: 11054
Uniprot ID: OGFR_HUMAN
Immunogen Region: 1-676
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-676 of human OGFR (NP_031372.2).
Immunogen Sequence: MDDPDCDSTWEEDEEDAEDA EDEDCEDGEAAGARDADAGD EDEESEEPRAARPSSFQSRM TGSRNWRATRDMCRYRHNYP DLVERDCNGDTPNLSFYRNE IRFLPNGCFIEDILQNWTDN YDLLEDNHSYIQWLFPLREP GVNWHAKPLTLREVEVFKSS QEIQERLVRAYELMLGFYGI RLEDRGTGTVGRAQNYQKRF QNLNWRSHNNLRITRILKSL GELGLEHFQAPLVRFFLEE
Tissue Specificity Highly expressed in the heart and liver, moderately in skeletal muscle and kidney and to a lesser extent in brain and pancreas. Expressed in fetal tissues including liver and kidney.
Function Receptor for opioid growth factor (OGF), also known as Met-enkephalin. Seems to be involved in growth regulation.
Protein Name Opioid Growth Factor Receptor
Ogfr
Protein 7-60
Zeta-Type Opioid Receptor
Cellular Localisation Cytoplasm
Nucleus
The Ogf/Ogfr Complex Is Probably Translocated To The Nucleus
Alternative Antibody Names Anti-Opioid Growth Factor Receptor antibody
Anti-Ogfr antibody
Anti-Protein 7-60 antibody
Anti-Zeta-Type Opioid Receptor antibody
Anti-OGFR antibody

Information sourced from Uniprot.org

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