STJ71544 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-OAZ antibody (N-Term) (STJ71544)

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SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71544-100
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General Information

Short DescriptionGoat polyclonal antibody anti-OAZ (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:2000.
IF: Strong expression of the protein seen in the nuclei of A431 cells. Recommended concentration: 10ยตg/ml.

FC:Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.<

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolZNF423
Gene ID23090
Uniprot IDZN423_HUMAN
Accession NumberNP_055884.2
Immunogen RegionN-Term
Immunogen SequenceEPECDQKTSRALEDR

Additional Info

Function Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation.this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
Protein Name Zinc Finger Protein 423
Olf1/Ebf-Associated Zinc Finger Protein
Hoaz
Smad- And Olf-Interacting Zinc Finger Protein
Database Links Reactome: R-HSA-9844594
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein 423 antibody
Anti-Olf1/Ebf-Associated Zinc Finger Protein antibody
Anti-Hoaz antibody
Anti-Smad- And Olf-Interacting Zinc Finger Protein antibody
Anti-ZNF423 antibody
Anti-KIAA0760 antibody
Anti-NPHP14 antibody
Anti-OAZ antibody

Information sourced from Uniprot.org

Citations

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