• STJ70993 (2µg/ml) staining of Human Thyroid lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Nur77/TR3-Isoform a antibody (Internal) (STJ70993)

SKU:
STJ70993-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Nur77/TR3-Isoform a (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IHC-5µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NR4A1
Gene ID: 3164
Uniprot ID: NR4A1_HUMAN
Immunogen Region: Internal
Accession Number: NP_002126.2; NP_775180.1
Specificity: This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.
Immunogen Sequence: GRLPSKPKQPPDAS
Post Translational Modifications Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli. Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1.
Function Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3'. Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter. May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration. Plays a role in the vascular response to injury. In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7.
Protein Name Nuclear Receptor Subfamily 4immunitygroup A Member 1
Early Response Protein Nak1
Nuclear Hormone Receptor Nur/77
Nur77
Orphan Nuclear Receptor Hmr
Orphan Nuclear Receptor Tr3
St-59
Testicular Receptor 3
Database Links Reactome: R-HSA-198693
Reactome: R-HSA-383280
Reactome: R-HSA-5674400
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Mitochondrion
Nuclear Export To The Cytosol Is Xpo1-Mediated And Positively Regulated By Ifi27
Translocation To The Mitochondrion Upon Interaction With Rxra And Upon The Presence Of 9-Cis Retinoic Acid
Alternative Antibody Names Anti-Nuclear Receptor Subfamily 4immunitygroup A Member 1 antibody
Anti-Early Response Protein Nak1 antibody
Anti-Nuclear Hormone Receptor Nur/77 antibody
Anti-Nur77 antibody
Anti-Orphan Nuclear Receptor Hmr antibody
Anti-Orphan Nuclear Receptor Tr3 antibody
Anti-St-59 antibody
Anti-Testicular Receptor 3 antibody
Anti-NR4A1 antibody
Anti-GFRP1 antibody
Anti-HMR antibody
Anti-NAK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance