| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Nur77/TR3-Isoform a (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 60kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3µg/ml. Primary incubation w |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NR4A1 |
| Gene ID: | 3164 |
| Uniprot ID: | NR4A1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_002126.2; NP_775180.1 |
| Specificity: | This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only. |
| Immunogen Sequence: | GRLPSKPKQPPDAS |
| Post Translational Modifications | Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli. Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1. |
| Function | Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3'. Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter. May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration. Plays a role in the vascular response to injury. In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7. |
| Protein Name | Nuclear Receptor Subfamily 4immunitygroup A Member 1 Early Response Protein Nak1 Nuclear Hormone Receptor Nur/77 Nur77 Orphan Nuclear Receptor Hmr Orphan Nuclear Receptor Tr3 St-59 Testicular Receptor 3 |
| Database Links | Reactome: R-HSA-198693 Reactome: R-HSA-383280 Reactome: R-HSA-5674400 |
| Cellular Localisation | Nucleus Cytoplasm Cytosol Mitochondrion Nuclear Export To The Cytosol Is Xpo1-Mediated And Positively Regulated By Ifi27 Translocation To The Mitochondrion Upon Interaction With Rxra And Upon The Presence Of 9-Cis Retinoic Acid |
| Alternative Antibody Names | Anti-Nuclear Receptor Subfamily 4immunitygroup A Member 1 antibody Anti-Early Response Protein Nak1 antibody Anti-Nuclear Hormone Receptor Nur/77 antibody Anti-Nur77 antibody Anti-Orphan Nuclear Receptor Hmr antibody Anti-Orphan Nuclear Receptor Tr3 antibody Anti-St-59 antibody Anti-Testicular Receptor 3 antibody Anti-NR4A1 antibody Anti-GFRP1 antibody Anti-HMR antibody Anti-NAK1 antibody |
Information sourced from Uniprot.org