• Immunofluorescence analysis of U-2 OS cells using NUP153 Rabbit monoclonal antibody (STJ11101828) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NUP153 in paraffin-embedded mouse testis tissue using NUP153 Rabbit monoclonal antibody (STJ11101828) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of NUP153 in paraffin-embedded human tonsil tissue using NUP153 Rabbit monoclonal antibody (STJ11101828) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of NUP153 in paraffin-embedded human cervix cancer tissue using NUP153 Rabbit monoclonal antibody (STJ11101828) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using NUP153 Rabbit monoclonal antibody (STJ11101828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NUP153 antibody (1-100) [S8MR] (STJ11101828)

SKU:
STJ11101828

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NUP153 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUP153
Gene ID: 9972
Uniprot ID: NU153_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUP153 (P49790).
Immunogen Sequence: MASGAGGVGGGGGGKIRTRR CHQGPIKPYQQGRQQHQGIL SRVTESVKNIVPGWLQRYFN KNEDVCSCSTDTSEVPRWPE NKEDHLVYADEESSNITDGR
Post Translational Modifications Phosphorylated in interphase, hyperphosphorylated during mitosis. May play a role in the reversible disassembly of the nuclear pore complex during mitosis. Proteolytically degraded after poliovirus (PV) infection.degradation is partial and NCP- and TPR-binding domains withstand degradation. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
Function Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus.in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC). (Microbial infection) Interacts with HIV-1 caspid protein P24 and thereby promotes the integration of the virus in the nucleus of non-dividing cells (in vitro). (Microbial infection) Binds HIV-2 protein vpx and thereby promotes the nuclear translocation of the lentiviral genome (in vitro).
Protein Name Nuclear Pore Complex Protein Nup153
153 Kda Nucleoporin
Nucleoporin Nup153
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-165054
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-168325
Reactome: R-HSA-168333
Reactome: R-HSA-170822
Reactome: R-HSA-180746
Reactome: R-HSA-180910
Reactome: R-HSA-191859
Reactome: R-HSA-3108214
Reactome: R-HSA-3232142
Reactome: R-HSA-3301854
Reactome: R-HSA-3371453
Reactome: R-HSA-4085377
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5578749
Reactome: R-HSA-5619107
Reactome: R-HSA-6784531
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9705671
Cellular Localisation Nucleus
Nucleus Membrane
Nuclear Pore Complex
Tightly Associated With The Nuclear Membrane And Lamina
Localized To The Nucleoplasmic Side Of The Nuclear Pore Complex (Npc) Core Structure
Forming A Fibrous Structure Called The Nuclear Basket
Dissociates From The Npc Structure Early During Prophase Of Mitosis
Integrated In The Newly Assembled Nuclear Envelope Of Postmitotic Cells Early In G1
Colocalized With Nup98 And Tpr To The Nuclear Basket At The Nucleoplasmic Side Of The Npc
Detected In Diffuse And Discrete Intranuclear Foci
Remained Localized To The Nuclear Membrane After Poliovirus (Pv) Infection
Alternative Antibody Names Anti-Nuclear Pore Complex Protein Nup153 antibody
Anti-153 Kda Nucleoporin antibody
Anti-Nucleoporin Nup153 antibody
Anti-NUP153 antibody

Information sourced from Uniprot.org

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