![Western blot analysis of lysates from wild type (WT) and MTH1 knockout (KO) 293T cells, using [KO Validated] MTH1 Rabbit polyclonal antibody (STJ115293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94325/375830/STJ115293_2__47263.1713142705.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MTH1 knockout (KO) 293T cells, using [KO Validated] MTH1 Rabbit polyclonal antibody (STJ115293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] MTH1 Rabbit polyclonal antibody (STJ115293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94325/375831/STJ115293_3__92206.1713142707.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] MTH1 Rabbit polyclonal antibody (STJ115293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
Anti-NUDT1 antibody (1-179) (STJ115293)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NUDT1 (1-179) antibody for use in WB, IP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NUDT1 |
| Gene ID | 4521 |
| Uniprot ID | 8ODP_HUMAN |
| Immunogen Region | 1-179 |
| Immunogen Sequence | MSGISPQQMGEPEGSWSGKN PGTMGASRLYTLVLVLQPQR VLLGMKKRGFGAGRWNGFGG KVQEGETIEDGARRELQEES GLTVDALHKVGQIVFEFVGE PELMDVHVFCTDSIQGTPVE SDEMRPCWFQLDQIPFKDMW PDDSYWFPLLLQKKKFHGYF KFQGQDTILDYTLREVDTV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MTH1 (NP_945192.1). |
Additional Info
| Tissue Specificity | Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes. |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Also has a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A. Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress. |
| Protein Name | Oxidized Purine Nucleoside Triphosphate Hydrolase2-Hydroxy-Datp Diphosphatase7 -8-Dihydro-8-Oxoguanine Triphosphatase8-Oxo-DgtpaseMethylated Purine Nucleoside Triphosphate HydrolaseNucleoside Diphosphate-Linked Moiety X Motif 1Nudix Motif 1 |
| Database Links | Reactome: R-HSA-2393930 |
| Cellular Localisation | Isoform P18: CytoplasmCytosolMitochondrion MatrixNucleusMostly Present In CytosolA Minor Proportion Is MitochondrialA Very Small Amount Of The Protein Is Associated With NucleiIsoform P26: Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Oxidized Purine Nucleoside Triphosphate Hydrolase antibodyAnti-2-Hydroxy-Datp Diphosphatase antibodyAnti-7 -8-Dihydro-8-Oxoguanine Triphosphatase antibodyAnti-8-Oxo-Dgtpase antibodyAnti-Methylated Purine Nucleoside Triphosphate Hydrolase antibodyAnti-Nucleoside Diphosphate-Linked Moiety X Motif 1 antibodyAnti-Nudix Motif 1 antibodyAnti-NUDT1 antibodyAnti-MTH1 antibody |
Information sourced from Uniprot.org