• Immunofluorescence analysis of U-2 OS cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and NUCB1 knockout (KO) 293T cells, using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of various lysates using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NUCB1 antibody (302-461) (STJ116225)

SKU:
STJ116225

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUCB1 (302-461) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUCB1
Gene ID: 4924
Uniprot ID: NUCB1_HUMAN
Immunogen Region: 302-461
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human NUCB1 (NP_006175.2).
Immunogen Sequence: KNVDTNQDRLVTLEEFLAST QRKEFGDTGEGWETVEMHPA YTEEELRRFEEELAAREAEL NAKAQRLSQETEALGRSQGR LEAQKRELQQAVLHMEQRKQ QQQQQQGHKAPAAHPEGQLK FHPDTDDVPVPAPAGDQKEV DTSEKKLLERLPEVEVPQHL
Tissue Specificity Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
Post Translational Modifications O-glycosylated.
Function Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis. Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins).
Protein Name Nucleobindin-1
Calnuc
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Golgi Apparatus
Cis-Golgi Network Membrane
Peripheral Membrane Protein
Lumenal Side
Cytoplasm
Secreted
A Small Fraction Of The Protein May Be Cytoplasmic
Alternative Antibody Names Anti-Nucleobindin-1 antibody
Anti-Calnuc antibody
Anti-NUCB1 antibody
Anti-NUC antibody

Information sourced from Uniprot.org

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