![Immunofluorescence analysis of U-2 OS cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95218/377088/STJ116225_1__84766.1713144314.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of L929 cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95218/377089/STJ116225_2__51466.1713144315.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and NUCB1 knockout (KO) 293T cells, using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95218/377090/STJ116225_3__53208.1713144315.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NUCB1 knockout (KO) 293T cells, using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western blot analysis of various lysates using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95218/377091/STJ116225_4__70360.1713144317.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/95218/377088/STJ116225_1__84766.1713144314.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] NUCB1 Rabbit polyclonal antibody (STJ116225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-NUCB1 antibody (302-461) (STJ116225)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NUCB1 (302-461) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NUCB1 |
| Gene ID | 4924 |
| Uniprot ID | NUCB1_HUMAN |
| Immunogen Region | 302-461 |
| Immunogen Sequence | KNVDTNQDRLVTLEEFLAST QRKEFGDTGEGWETVEMHPA YTEEELRRFEEELAAREAEL NAKAQRLSQETEALGRSQGR LEAQKRELQQAVLHMEQRKQ QQQQQQGHKAPAAHPEGQLK FHPDTDDVPVPAPAGDQKEV DTSEKKLLERLPEVEVPQHL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human NUCB1 (NP_006175.2). |
Additional Info
| Tissue Specificity | Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas. |
| Post Translational Modifications | O-glycosylated. |
| Function | Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis. Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins). |
| Protein Name | Nucleobindin-1Calnuc |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Golgi ApparatusCis-Golgi Network MembranePeripheral Membrane ProteinLumenal SideCytoplasmSecretedA Small Fraction Of The Protein May Be Cytoplasmic |
| Alternative Antibody Names | Anti-Nucleobindin-1 antibodyAnti-Calnuc antibodyAnti-NUCB1 antibodyAnti-NUC antibody |
Information sourced from Uniprot.org