• Western blot analysis of various lysates using NSMF Rabbit polyclonal antibody (STJ24737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NSMF antibody (226-505) (STJ24737)

SKU:
STJ24737

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NSMF (226-505) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSMF
Gene ID: 26012
Uniprot ID: NSMF_HUMAN
Immunogen Region: 226-505
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 226-505 of human NSMF (NP_001124443.1).
Immunogen Sequence: FQTATTTMQANFRKHLRMVG SRRVKAQTFAERRERSFSRS WSDPTPMKADTSHDSRDSSD LQSSHCTLDEAFEDLDWDTE KGLEAVACDTEGFVPPKVML ISSKVPKAEYIPTIIRRDDP SIIPILYDHEHATFEDILEE IERKLNVYHKGAKIWKMLIF CQGGPGHLYLLKNKVATFAK VEKEEDMIHFWKRLSRLMSK VNPEPNVIHIMGCYILGNPN GEKLFQNLRTLMTPYRVTF
Tissue Specificity Highly expressed in adult and fetal brain. Weakly expressed in heart, liver, spleen, testis, small intestine, skeletal muscle, peripheral white blood cells and kidney.
Post Translational Modifications Proteolytically processed after NMDA receptor activation. Cleaved in a calcium-dependent and calpain-sensitive manner. Calpain cleavage is essential for the translocation process from dendrites to the nucleus.
Function Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells.
Protein Name Nmda Receptor Synaptonuclear Signaling And Neuronal Migration Factor
Nasal Embryonic Luteinizing Hormone-Releasing Hormone Factor
Nasal Embryonic Lhrh Factor
Cellular Localisation Nucleus
Nucleus Envelope
Nucleus Membrane
Nucleus Matrix
Cytoplasm
Cell Cortex
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Cell Projection
Dendrite
Synapse
Synaptosome
Postsynaptic Density
Membrane
Found On The Outside Of The Luteinizing-Hormone-Releasing Hormone (Lhrh) Cell Membrane And Axons Projecting From The Olfactory Pit And Epithelium
Associates With Transcriptionally Active Chromatin Regions
Detected At The Nuclear Membranes Of Ca1 Neurons
Cortical Cytoskeleton
Localized In Proximal Apical Dendrites
Colocalizes With Cabp1 In Dendrites And Dendritic Spines
Myristoylation Is A Prerequisite For Extranuclear Localization
Translocates From Dendrites To The Nucleus During Nmda Receptor-Dependent Long-Term Potentiation (Ltp) Induction Of Synaptic Transmission At Schaffer Collateral/Ca1 Synapses Of Hippocampal Primary Neurons And In A Importin-Dependent Manner
Alternative Antibody Names Anti-Nmda Receptor Synaptonuclear Signaling And Neuronal Migration Factor antibody
Anti-Nasal Embryonic Luteinizing Hormone-Releasing Hormone Factor antibody
Anti-Nasal Embryonic Lhrh Factor antibody
Anti-NSMF antibody
Anti-NELF antibody

Information sourced from Uniprot.org

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