• Western blot analysis of extracts of MCF7 cells, using NSD1 antibody (STJ112022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NSD1 antibody (60-2696) (STJ112022)

SKU:
STJ112022

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NSD1 (60-2696) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSD1
Gene ID: 64324
Uniprot ID: NSD1_HUMAN
Immunogen Region: 60-2696
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-2696 of human NSD1 (NP_071900.2).
Immunogen Sequence: AYGQDSPSCYIPLRRLQDLA SMINVEYLNGSADGSESFQD PEKSDSRAQTPIVCTSLSPG GPTALAMKQEPSCNNSPELQ VKVTKTIKNGFLHFENFTCV DDADVDSEMDPEQPVTEDES IEEIFEETQTNATCNYETKS ENGVKVAMGSEQDSTPESRH GAVKSPFLPLAPQTETQKNK QRNEVDGSNEKAALLPAPFS L
Tissue Specificity Expressed in the fetal/adult brain, kidney, skeletal muscle, spleen, and the thymus, and faintly in the lung.
Function Histone methyltransferase that dimethylates Lys-36 of histone H3 (H3K36me2). Transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context.
Protein Name Histone-Lysine N-Methyltransferase - H3 Lysine-36 Specific
Androgen Receptor Coactivator 267 Kda Protein
Androgen Receptor-Associated Protein Of 267 Kda
H3-K36-Hmtase
Lysine N-Methyltransferase 3b
Nuclear Receptor-Binding Set Domain-Containing Protein 1
Nr-Binding Set Domain-Containing Protein
Database Links Reactome: R-HSA-3214841
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase - H3 Lysine-36 Specific antibody
Anti-Androgen Receptor Coactivator 267 Kda Protein antibody
Anti-Androgen Receptor-Associated Protein Of 267 Kda antibody
Anti-H3-K36-Hmtase antibody
Anti-Lysine N-Methyltransferase 3b antibody
Anti-Nuclear Receptor-Binding Set Domain-Containing Protein 1 antibody
Anti-Nr-Binding Set Domain-Containing Protein antibody
Anti-NSD1 antibody
Anti-ARA267 antibody
Anti-KMT3B antibody

Information sourced from Uniprot.org

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