• Immunofluorescence analysis of NIH-3T3 cells using Mineralocorticoid receptor (NR3C2) Rabbit polyclonal antibody (STJ116199) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Mineralocorticoid receptor (NR3C2) Rabbit polyclonal antibody (STJ116199) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Mineralocorticoid receptor (NR3C2) in paraffin-embedded human liver cancer using Mineralocorticoid receptor (NR3C2) Rabbit polyclonal antibody (STJ116199) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Mineralocorticoid receptor (NR3C2) Rabbit polyclonal antibody (STJ116199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NR3C2 antibody (450-600) (STJ116199)

SKU:
STJ116199

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NR3C2 (450-600) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR3C2
Gene ID: 4306
Uniprot ID: MCR_HUMAN
Immunogen Region: 450-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 450-600 of human Mineralocorticoid receptor (NR3C2) (NP_000892.2).
Immunogen Sequence: FPFMDGSYFSFMDDKDYYSL SGILGPPVPGFDGNCEGSGF PVGIKQEPDDGSYYPEASIP SSAIVGVNSGGQSFHYRIGA QGTISLSRSARDQSFQHLSS FPPVNTLVESWKSHGDLSSR RSDGYPVLEYIPENVSSSTL RSVSTGSSRPS
Tissue Specificity Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
Post Translational Modifications Phosphorylated.
Function Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
Protein Name Mineralocorticoid Receptor
Mr
Nuclear Receptor Subfamily 3 Group C Member 2
Database Links Reactome: R-HSA-3371497
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Cellular Localisation Cytoplasm
Nucleus
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic And Nuclear In The Absence Of Ligand
Nuclear After Ligand-Binding
When Bound To Hsd11b2
It Is Found Associated With The Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Mineralocorticoid Receptor antibody
Anti-Mr antibody
Anti-Nuclear Receptor Subfamily 3 Group C Member 2 antibody
Anti-NR3C2 antibody
Anti-MCR antibody
Anti-MLR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance