Immunohistochemistry analysis ofย NR2F1 in paraffin-embeddedย human tonsil tissue usingย NR2F1 Rabbit polyclonal antibody (STJ11103861) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-NR2F1 antibody (60-180) (STJ11103861)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103861
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General Information

Short DescriptionRabbit polyclonal Coup Transcription Factor 1 (60-180) antibody for use in IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC-P/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeIHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNR2F1
Gene ID7025
Uniprot IDCOT1_HUMAN
Immunogen Region60-180
Immunogen SequenceAPATPGTAGDKGQGPPGSGQ SQQHIECVVCGDKSSGKHYG QFTCEGCKSFFKRSVRRNLT YTCRANRNCPIDQHHRNQCQ YCRLKKCLKVGMRREAVQRG RMPPTQPNPGQYALTNGDPL N
SpecificityA synthetic peptide corresponding to a sequence within amino acids 60-180 of human NR2F1 (NP_005645.1).

Additional Info

Function Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
Protein Name Coup Transcription Factor 1
Coup-Tf1
Coup Transcription Factor I
Coup-Tf I
Nuclear Receptor Subfamily 2 Group F Member 1
V-Erba-Related Protein 3
Ear-3
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Coup Transcription Factor 1 antibody
Anti-Coup-Tf1 antibody
Anti-Coup Transcription Factor I antibody
Anti-Coup-Tf I antibody
Anti-Nuclear Receptor Subfamily 2 Group F Member 1 antibody
Anti-V-Erba-Related Protein 3 antibody
Anti-Ear-3 antibody
Anti-NR2F1 antibody
Anti-EAR3 antibody
Anti-ERBAL3 antibody
Anti-TFCOUP1 antibody

Information sourced from Uniprot.org

Citations

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