• Immunoprecipitation of NR1I3 in 300 µg extracts from 293T cells transfected with NR1I3 using 3 µg NR1I3 Rabbit polyclonal antibody (STJ26189). Western blot analysis was performed using NR1I3 Rabbit polyclonal antibody (STJ26189) at 1:1000 dilution.
  • Immunofluorescence analysis of HepG2 cells using NR1I3 Rabbit polyclonal antibody (STJ26189) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NR1I3 Rabbit polyclonal antibody (STJ26189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NR1I3 antibody (103-352) (STJ26189)

SKU:
STJ26189

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NR1I3 (103-352) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR1I3
Gene ID: 9970
Uniprot ID: NR1I3_HUMAN
Immunogen Region: 103-352
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 103-352 of human NR1I3 (NP_001070948.1).
Immunogen Sequence: PVQLSKEQEELIRTLLGAHT RHMGTMFEQFVQFRPPAHLF IHHQPLPTLAPVLPLVTHFA DINTFMVLQVIKFTKDLPVF RSLPIEDQISLLKGAAVEIC HIVLNTTFCLQTQNFLCGPL RYTIEDGARVSPTVGFQVEF LELLFHFHGTLRKLQLQEPE YVLLAAMALFSPDRPGVTQR DEIDQLQEEMALTLQSYIKG QQRRPRDRFLYAKLLGLLAE LRSINEAYGYQIQHIQGLS
Tissue Specificity Predominantly expressed in liver.
Post Translational Modifications Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.
Function Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
Protein Name Nuclear Receptor Subfamily 1 Group I Member 3
Constitutive Activator Of Retinoid Response
Constitutive Active Response
Constitutive Androstane Receptor
Car
Orphan Nuclear Receptor Mb67
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Recruited To The Cytoplasm By Dnajc7
Alternative Antibody Names Anti-Nuclear Receptor Subfamily 1 Group I Member 3 antibody
Anti-Constitutive Activator Of Retinoid Response antibody
Anti-Constitutive Active Response antibody
Anti-Constitutive Androstane Receptor antibody
Anti-Car antibody
Anti-Orphan Nuclear Receptor Mb67 antibody
Anti-NR1I3 antibody
Anti-CAR antibody

Information sourced from Uniprot.org

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