Anti-NR0B2 antibody (40-124) (STJ118882)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NR0B2 (40-124) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NR0B2 |
| Gene ID | 8431 |
| Uniprot ID | NR0B2_HUMAN |
| Immunogen Region | 40-124 |
| Immunogen Sequence | LCRQHRPVQLCAPHRTCREA LDVLAKTVAFLRNLPSFWQL PPQDQRRLLQGCWGPLFLLG LAQDAVTFEVAEAPVPSILK KILLE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 40-124 of human SHP/NR0B2 (NP_068804.1). |
Additional Info
| Tissue Specificity | Liver. Low levels of expression were detected in heart and pancreas. |
| Post Translational Modifications | Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors. |
| Function | Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with which it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1. Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism. Regulates the circadian expression of cytochrome P450 (CYP) enzymes. Represses: NR5A2 and HNF4A to down-regulate CYP2C38, NFLI3 to up-regulate CYP2A5, BHLHE41/HNF1A axis to up-regulate CYP1A2, CYP2E1 and CYP3A11, and NR1D1 to up-regulate CYP2B10, CYP4A10 and CYP4A14. |
| Protein Name | Nuclear Receptor Subfamily 0 Group B Member 2Orphan Nuclear Receptor ShpSmall Heterodimer Partner |
| Database Links | Reactome: R-HSA-383280 |
| Cellular Localisation | NucleusCytoplasmColocalizes With Neurod1 In The Nucleus |
| Alternative Antibody Names | Anti-Nuclear Receptor Subfamily 0 Group B Member 2 antibodyAnti-Orphan Nuclear Receptor Shp antibodyAnti-Small Heterodimer Partner antibodyAnti-NR0B2 antibodyAnti-SHP antibody |
Information sourced from Uniprot.org