• Western blot analysis of extracts of various cell lines, using SHP/NR0B2 antibody (STJ118882) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NR0B2 antibody (40-124) (STJ118882)

SKU:
STJ118882

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NR0B2 (40-124) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR0B2
Gene ID: 8431
Uniprot ID: NR0B2_HUMAN
Immunogen Region: 40-124
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-124 of human SHP/NR0B2 (NP_068804.1).
Immunogen Sequence: LCRQHRPVQLCAPHRTCREA LDVLAKTVAFLRNLPSFWQL PPQDQRRLLQGCWGPLFLLG LAQDAVTFEVAEAPVPSILK KILLE
Tissue Specificity Liver. Low levels of expression were detected in heart and pancreas.
Post Translational Modifications Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
Function Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with which it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1. Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism. Regulates the circadian expression of cytochrome P450 (CYP) enzymes. Represses: NR5A2 and HNF4A to down-regulate CYP2C38, NFLI3 to up-regulate CYP2A5, BHLHE41/HNF1A axis to up-regulate CYP1A2, CYP2E1 and CYP3A11, and NR1D1 to up-regulate CYP2B10, CYP4A10 and CYP4A14.
Protein Name Nuclear Receptor Subfamily 0 Group B Member 2
Orphan Nuclear Receptor Shp
Small Heterodimer Partner
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Cytoplasm
Colocalizes With Neurod1 In The Nucleus
Alternative Antibody Names Anti-Nuclear Receptor Subfamily 0 Group B Member 2 antibody
Anti-Orphan Nuclear Receptor Shp antibody
Anti-Small Heterodimer Partner antibody
Anti-NR0B2 antibody
Anti-SHP antibody

Information sourced from Uniprot.org

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