| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:10-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NR0B1 |
| Gene ID: | 190 |
| Uniprot ID: | NR0B1_HUMAN |
| Immunogen Region: | 1-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human NR0B1 (NP_000466.2). |
| Immunogen Sequence: | MAGENHQWQGSILYNMLMSA KQTRAAPEAPETRLVDQCWG CSCGDEPGVGREGLLGGRNV ALLYRCCFCGKDHPRQGSIL YSMLTSAKQTYAAPKAPEAT LGPCWGCSCGSDPGVGRAGL PGGRPVALLYRCCFCGEDHP RQGSILYSLLTSSKQTHVAP AAPEARPGGAWWDRSYFAQR PGGKEALPGGRATALLYRCC FCGEDHPQQGSTLYCVPTST NQAQAAPEERPRAPWWDTS |
| Function | Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. |
| Protein Name | Nuclear Receptor Subfamily 0 Group B Member 1Dss-Ahc Critical Region On The X Chromosome Protein 1Nuclear Receptor Dax-1 |
| Database Links | Reactome: R-HSA-383280 |
| Cellular Localisation | NucleusCytoplasmShuttles Between The Cytoplasm And NucleusHomodimers Exits In The Cytoplasm And In The Nucleus |
| Alternative Antibody Names | Anti-Nuclear Receptor Subfamily 0 Group B Member 1 antibodyAnti-Dss-Ahc Critical Region On The X Chromosome Protein 1 antibodyAnti-Nuclear Receptor Dax-1 antibodyAnti-NR0B1 antibodyAnti-AHC antibodyAnti-DAX1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
