Anti-NPC1 antibody (1228-1278) (STJ501954)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 1228-1278 on human Niemann-Pick C1 protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-NPC1 (1228-1278) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 µg/µl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500ELISA: 1:4, 000IP: 1:150IHC: 1:100ICC: 1:100IF: 1:100 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | NPC1 |
| Gene ID | 4864 |
| Uniprot ID | NPC1_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 1228-1278 on human Niemann-Pick C1 protein. |
| Immunogen Region | 1228-1278 |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). Inhibits cholesterol-mediated mTORC1 activation throught its interaction with SLC38A9. (Microbial infection) Acts as an endosomal entry receptor for ebolavirus. |
| Protein Name | Npc Intracellular Cholesterol Transporter 1Niemann-Pick C1 Protein |
| Database Links | Reactome: R-HSA-8964038 |
| Cellular Localisation | Late Endosome MembraneMulti-Pass Membrane ProteinLysosome Membrane |
| Alternative Antibody Names | Anti-Npc Intracellular Cholesterol Transporter 1 antibodyAnti-Niemann-Pick C1 Protein antibodyAnti-NPC1 antibody |
Information sourced from Uniprot.org