• Immunofluorescence analysis of NIH/3T3 cells using NPB Rabbit polyclonal antibody (STJ119039) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using NPB Rabbit polyclonal antibody (STJ119039) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NPB Rabbit polyclonal antibody (STJ119039) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NPB antibody (1-125) (STJ119039)

SKU:
STJ119039

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NPB (1-125) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPB
Gene ID: 256933
Uniprot ID: NPB_HUMAN
Immunogen Region: 1-125
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human NPB (NP_683694.1).
Immunogen Sequence: MARSATLAAAALALCLLLAP PGLAWYKPAAGHSSYSVGRA AGLLSGLRRSPYARRSQPYR GAEPPGGAGASPELQLHPRL RSLAVCVQDVAPNLQRCERL PDGRGTYQCKANVFLSLRAA DCLAA
Tissue Specificity Widely expressed in the central nervous system. High levels are found in substantia nigra, hypothalamus, hippocampus, spinal cord, placenta and fetal brain.lower levels are found in testis, uterus and ovary. Also detected at high levels in colorectal adenocarcinoma.
Function May be involved in the regulation of feeding, neuroendocrine system, memory, learning and in the afferent pain pathway.
Protein Name Neuropeptide B
Preproprotein L7
Hppl7 Cleaved Into - Neuropeptide B-23
Npb23
Hl7 - Neuropeptide B-29
Npb29
Hl7c
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative Antibody Names Anti-Neuropeptide B antibody
Anti-Preproprotein L7 antibody
Anti-Hppl7 Cleaved Into - Neuropeptide B-23 antibody
Anti-Npb23 antibody
Anti-Hl7 - Neuropeptide B-29 antibody
Anti-Npb29 antibody
Anti-Hl7c antibody
Anti-NPB antibody
Anti-PPL7 antibody
Anti-PPNPB antibody

Information sourced from Uniprot.org

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