Anti-NGEF antibody (429-710) (STJ118934)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NGEF (429-710) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NGEF |
| Gene ID | 25791 |
| Uniprot ID | NGEF_HUMAN |
| Immunogen Region | 429-710 |
| Immunogen Sequence | RVEERSERECTALDAHKELE MVVKACNEGVRKMSRTEQMI SIQKKMEFKIKSVPIISHSR WLLKQGELQQMSGPKTSRTL RTKKLFHEIYLFLFNDLLVI CRQIPGDKYQVFDSAPRGLL RVEELEDQGQTLANVFILRL LENADDREATYMLKASSQSE MKRWMTSLAPNRRTKFVSFT SRLLDCPQVQCVHPYVAQQP DELTLELADILNILDKTDDG WIFGERLHDQERGWFPSSM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 429-710 of human NGEF (NP_062824.2). |
Additional Info
| Tissue Specificity | Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. |
| Post Translational Modifications | Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. Phosphorylation by CDK5 upon EPHA4 activation by EFNA1 may regulate dendritic spine morphogenesis. |
| Function | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. |
| Protein Name | Ephexin-1Eph-Interacting Exchange ProteinNeuronal Guanine Nucleotide Exchange Factor |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-3928663Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | CytoplasmMembraneCell ProjectionGrowth ConeAssociated With MembranesLocalizes To Axonal Growth Cones |
| Alternative Antibody Names | Anti-Ephexin-1 antibodyAnti-Eph-Interacting Exchange Protein antibodyAnti-Neuronal Guanine Nucleotide Exchange Factor antibodyAnti-NGEF antibody |
Information sourced from Uniprot.org