| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NGEF (429-710) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NGEF |
| Gene ID: |
25791 |
| Uniprot ID: |
NGEF_HUMAN |
| Immunogen Region: |
429-710 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 429-710 of human NGEF (NP_062824.2). |
| Immunogen Sequence: |
RVEERSERECTALDAHKELE MVVKACNEGVRKMSRTEQMI SIQKKMEFKIKSVPIISHSR WLLKQGELQQMSGPKTSRTL RTKKLFHEIYLFLFNDLLVI CRQIPGDKYQVFDSAPRGLL RVEELEDQGQTLANVFILRL LENADDREATYMLKASSQSE MKRWMTSLAPNRRTKFVSFT SRLLDCPQVQCVHPYVAQQP DELTLELADILNILDKTDDG WIFGERLHDQERGWFPSSM |
| Tissue Specificity | Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. |
| Post Translational Modifications | Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. Phosphorylation by CDK5 upon EPHA4 activation by EFNA1 may regulate dendritic spine morphogenesis. |
| Function | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. |
| Protein Name | Ephexin-1Eph-Interacting Exchange ProteinNeuronal Guanine Nucleotide Exchange Factor |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-3928663Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | CytoplasmMembraneCell ProjectionGrowth ConeAssociated With MembranesLocalizes To Axonal Growth Cones |
| Alternative Antibody Names | Anti-Ephexin-1 antibodyAnti-Eph-Interacting Exchange Protein antibodyAnti-Neuronal Guanine Nucleotide Exchange Factor antibodyAnti-NGEF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
