Western blot analysis of lysates from Rat thymus, using NFYC Rabbit pAb (STJ111874) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NFYC antibody (27-335) (STJ111874)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111874
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit polyclonal NFYC (27-335) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityRat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNFYC
Gene ID4802
Uniprot IDNFYC_HUMAN
Immunogen Region27-335
Immunogen SequenceMEEIRNLTVKDFRVQELPLA RIKKIMKLDEDVKMISAEAP VLFAKAAQIFITELTLRAWI HTEDNKRRTLQRNDIAMAIT KFDQFDFLIDIVPR
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 27-120 of human NFYC (NP_055038.2).

Additional Info

Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Protein Name Nuclear Transcription Factor Y Subunit Gamma
Caat Box Dna-Binding Protein Subunit C
Nuclear Transcription Factor Y Subunit C
Nf-Yc
Transactivator Hsm-1/2
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2426168
Reactome: R-HSA-380994
Reactome: R-HSA-381183
Reactome: R-HSA-9614657
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Transcription Factor Y Subunit Gamma antibody
Anti-Caat Box Dna-Binding Protein Subunit C antibody
Anti-Nuclear Transcription Factor Y Subunit C antibody
Anti-Nf-Yc antibody
Anti-Transactivator Hsm-1/2 antibody
Anti-NFYC antibody

Information sourced from Uniprot.org

Citations

Product Review

!