• Western blot analysis of extracts of various cell lines, using NFYC antibody (STJ111874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of Rat thymus, using NFYC antibody (STJ111874) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NFYC antibody (27-335) (STJ111874)

SKU:
STJ111874

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFYC
Gene ID: 4802
Uniprot ID: NFYC_HUMAN
Immunogen Region: 27-335
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 27-120 of human NFYC (NP_055038.2).
Immunogen Sequence: MEEIRNLTVKDFRVQELPLA RIKKIMKLDEDVKMISAEAP VLFAKAAQIFITELTLRAWI HTEDNKRRTLQRNDIAMAIT KFDQFDFLIDIVPR
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Protein Name Nuclear Transcription Factor Y Subunit Gamma
Caat Box Dna-Binding Protein Subunit C
Nuclear Transcription Factor Y Subunit C
Nf-Yc
Transactivator Hsm-1/2
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2426168
Reactome: R-HSA-380994
Reactome: R-HSA-381183
Reactome: R-HSA-9614657
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Transcription Factor Y Subunit Gamma antibody
Anti-Caat Box Dna-Binding Protein Subunit C antibody
Anti-Nuclear Transcription Factor Y Subunit C antibody
Anti-Nf-Yc antibody
Anti-Transactivator Hsm-1/2 antibody
Anti-NFYC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance