• Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody (STJ115964). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse liver, using NFIL3 antibody (STJ115964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-NFIL3 antibody (133-462) (STJ115964)

SKU:
STJ115964

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFIL3 (133-462) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFIL3
Gene ID: 4783
Uniprot ID: NFIL3_HUMAN
Immunogen Region: 133-462
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2).
Immunogen Sequence: AYAQEIQKLSNSTAVYFQDY QTSKSNVSSFVDEHEPSMVS SSCISVIKHSPQSSLSDVSE VSSVEHTQESSVQGSCRSPE NKFQIIKQEPMELESYTREP RDDRGSYTASIYQNYMGNSF SGYSHSPPLLQVNRSSSNSP RTSETDDGVVGKSSDGEDEQ QVPKGPIHSPVELKHVHATV VKVPEVNSSALPHKLRIKAK AMQIKVEAFDNEFEATQKLS SPIDMTSKRHFELEKHSAP
Tissue Specificity Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
Function Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-GATTACTGTAACT-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Represses the transcription of CYP2A5. Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2. Required for the development of natural killer cell precursors.
Protein Name Nuclear Factor Interleukin-3-Regulated Protein
E4 Promoter-Binding Protein 4
Interleukin-3 Promoter Transcriptional Activator
Interleukin-3-Binding Protein 1
Transcriptional Activator Nf-Il3a
Database Links Reactome: R-HSA-400253
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor Interleukin-3-Regulated Protein antibody
Anti-E4 Promoter-Binding Protein 4 antibody
Anti-Interleukin-3 Promoter Transcriptional Activator antibody
Anti-Interleukin-3-Binding Protein 1 antibody
Anti-Transcriptional Activator Nf-Il3a antibody
Anti-NFIL3 antibody
Anti-E4BP4 antibody
Anti-IL3BP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance