| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Nesprin 1 (8220-8410) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SYNE1 |
| Gene ID: |
23345 |
| Uniprot ID: |
SYNE1_HUMAN |
| Immunogen Region: |
8220-8410 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 8220-8410 of human Nesprin 1 (NP_892006.3). |
| Immunogen Sequence: |
HDLSDRELELEDSAALSDLH WHDRSADSLLSPQPSSNLSL SLAQPLRSERSGRDTPASVD SIPLEWDHDYDLSRDLESAM SRALPSEDEEGQDDKDFYLR GAVGLSGDHSALESQIRQLG KALDDSRFQIQQTENIIRSK TPTGPELDTSYKGYMKLLGE CSSSIDSVKRLEHKLKEEEE SLPGFVNLHST |
| Tissue Specificity | Expressed in HeLa, A431, A172 and HaCaT cells (at protein level). Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, peripheral blood leukocytes, pancreas, cerebellum, stomach, kidney and placenta. Isoform GSRP-56 is predominantly expressed in heart and skeletal muscle (at protein level). |
| Post Translational Modifications | The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces. |
| Function | Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes.SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenesis.a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette. |
| Protein Name | Nesprin-1EnaptinKash Domain-Containing Protein 1Kash1Myocyte Nuclear Envelope Protein 1Myne-1Nuclear Envelope Spectrin Repeat Protein 1Synaptic Nuclear Envelope Protein 1Syne-1 |
| Database Links | Reactome: R-HSA-1221632 |
| Cellular Localisation | Nucleus Outer MembraneSingle-Pass Type Iv Membrane ProteinCytoplasmic SideNucleusNucleus EnvelopeCytoplasmCytoskeletonMyofibrilSarcomereThe Largest Part Of The Protein Is CytoplasmicWhile Its C-Terminal Part Is Associated With The Nuclear EnvelopeMost Probably The Outer Nuclear MembraneIn Skeletal And Smooth MusclesA Significant Amount Is Found In The SarcomeresIn MyoblastsRelocalized From The Nuclear Envelope To The Nucleus And Cytoplasm During Cell DifferentiationIsoform Gsrp-56: Golgi Apparatus |
| Alternative Antibody Names | Anti-Nesprin-1 antibodyAnti-Enaptin antibodyAnti-Kash Domain-Containing Protein 1 antibodyAnti-Kash1 antibodyAnti-Myocyte Nuclear Envelope Protein 1 antibodyAnti-Myne-1 antibodyAnti-Nuclear Envelope Spectrin Repeat Protein 1 antibodyAnti-Synaptic Nuclear Envelope Protein 1 antibodyAnti-Syne-1 antibodyAnti-SYNE1 antibodyAnti-C6orf98 antibodyAnti-KIAA0796 antibodyAnti-KIAA1262 antibodyAnti-KIAA1756 antibodyAnti-MYNE1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
