Anti-NEK9 antibody [R03-1F1] (STJA0034627)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human NEK9
General Information
| Short Description | Rabbit monoclonal anti-NEK9 for use in WB, IHC-F, IHC-P, ICC and IF in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-1F1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | NEK9 |
| Gene ID | 91754 |
| Uniprot ID | NEK9_HUMAN |
| Immunogen | A synthetic peptide of human NEK9 |
Additional Info
| Tissue Specificity | Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts. |
| Post Translational Modifications | Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. |
| Function | Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. |
| Protein Name | Serine/Threonine-Protein Kinase Nek9Nercc1 KinaseNever In Mitosis A-Related Kinase 9Nima-Related Protein Kinase 9Nima-Related Kinase 8Nek8 |
| Database Links | Reactome: R-HSA-2980767Reactome: R-HSA-3301854Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek9 antibodyAnti-Nercc1 Kinase antibodyAnti-Never In Mitosis A-Related Kinase 9 antibodyAnti-Nima-Related Protein Kinase 9 antibodyAnti-Nima-Related Kinase 8 antibodyAnti-Nek8 antibodyAnti-NEK9 antibodyAnti-KIAA1995 antibodyAnti-NEK8 antibodyAnti-NERCC antibody |
Information sourced from Uniprot.org