• Western blot analysis of extracts of various cell lines, using NEK7 antibody (STJ11104174) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using NEK7 antibody (STJ11104174) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NEK7 antibody (257-301) (STJ11104174)

SKU:
STJ11104174

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine/Threonine-Protein Kinase Nek7 (257-301) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEK7
Gene ID: 140609
Uniprot ID: NEK7_HUMAN
Immunogen Region: 257-301
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 257-301 of human NEK7 (NP_598001.1).
Immunogen Sequence: PLPSDHYSEELRQLVNMCIN PDPEKRPDVTYVYDVAKRMH ACTAS
Tissue Specificity Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
Post Translational Modifications Phosphorylation at Ser-195 required for its activation.
Function Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. Phosphorylates RPS6KB1. Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity. Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis.
Protein Name Serine/Threonine-Protein Kinase Nek7
Never In Mitosis A-Related Kinase 7
Nima-Related Protein Kinase 7
Database Links Reactome: R-HSA-2980767
Reactome: R-HSA-3301854
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle Pole
Microtubule Organizing Center
Centrosome
Present At Centrosome Throughout The Cell Cycle
Also Detected At Spindle Midzone Of The Anaphase Cells And Eventually Concentrates At The Midbody
Interaction With Anks3 Prevents Its Translocation To The Nucleus
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek7 antibody
Anti-Never In Mitosis A-Related Kinase 7 antibody
Anti-Nima-Related Protein Kinase 7 antibody
Anti-NEK7 antibody

Information sourced from Uniprot.org

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