Anti-NEK7 antibody (257-301) (STJ11104174)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Serine/Threonine-Protein Kinase Nek7 (257-301) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NEK7 |
| Gene ID | 140609 |
| Uniprot ID | NEK7_HUMAN |
| Immunogen Region | 257-301 |
| Immunogen Sequence | PLPSDHYSEELRQLVNMCIN PDPEKRPDVTYVYDVAKRMH ACTAS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 257-301 of human NEK7 (NP_598001.1). |
Additional Info
| Tissue Specificity | Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. |
| Post Translational Modifications | Phosphorylation at Ser-195 required for its activation. |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. Phosphorylates RPS6KB1. Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity. Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis. |
| Protein Name | Serine/Threonine-Protein Kinase Nek7Never In Mitosis A-Related Kinase 7Nima-Related Protein Kinase 7 |
| Database Links | Reactome: R-HSA-2980767Reactome: R-HSA-3301854Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomePresent At Centrosome Throughout The Cell CycleAlso Detected At Spindle Midzone Of The Anaphase Cells And Eventually Concentrates At The MidbodyInteraction With Anks3 Prevents Its Translocation To The Nucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek7 antibodyAnti-Never In Mitosis A-Related Kinase 7 antibodyAnti-Nima-Related Protein Kinase 7 antibodyAnti-NEK7 antibody |
Information sourced from Uniprot.org