| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Serine/Threonine-Protein Kinase Nek7 (257-301) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NEK7 |
| Gene ID: |
140609 |
| Uniprot ID: |
NEK7_HUMAN |
| Immunogen Region: |
257-301 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 257-301 of human NEK7 (NP_598001.1). |
| Immunogen Sequence: |
PLPSDHYSEELRQLVNMCIN PDPEKRPDVTYVYDVAKRMH ACTAS |
| Tissue Specificity | Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. |
| Post Translational Modifications | Phosphorylation at Ser-195 required for its activation. |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. Phosphorylates RPS6KB1. Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity. Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis. |
| Protein Name | Serine/Threonine-Protein Kinase Nek7Never In Mitosis A-Related Kinase 7Nima-Related Protein Kinase 7 |
| Database Links | Reactome: R-HSA-2980767Reactome: R-HSA-3301854Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomePresent At Centrosome Throughout The Cell CycleAlso Detected At Spindle Midzone Of The Anaphase Cells And Eventually Concentrates At The MidbodyInteraction With Anks3 Prevents Its Translocation To The Nucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek7 antibodyAnti-Never In Mitosis A-Related Kinase 7 antibodyAnti-Nima-Related Protein Kinase 7 antibodyAnti-NEK7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
