• Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.
  • Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (STJA0003701, 1:5000, red) and anti-Neurofilament-H ( cat. STJA0003698, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
  • Immunostaining of cultured rat neurons showing labeling of NF-M (STJA0003701, green, 1:100) in mature neurons.

Anti-NEFM antibody (C-Term) [3H11] (STJA0003701)

SKU:
STJA0003701-100

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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Bovine/Chicken/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-NEFM/NF-M (C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 3H11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 10 mM Sodium Azide.
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:1000
IHC 1:500-1:5000
ICC 1:100-1:500
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Nefm
Gene ID: 24588
Uniprot ID: NFM_RAT
Immunogen Region: C-Term
Immunogen: Preparation containing the extreme C-terminus expressed in and purification from E. Coli.
Tissue Specificity Expressed in the dorsal root ganglion neurons (at protein level).
Post Translational Modifications There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Medium Polypeptide
Nf-M
160 Kda Neurofilament Protein
Neurofilament 3
Neurofilament Triplet M Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Axon
Alternative Antibody Names Anti-Neurofilament Medium Polypeptide antibody
Anti-Nf-M antibody
Anti-160 Kda Neurofilament Protein antibody
Anti-Neurofilament 3 antibody
Anti-Neurofilament Triplet M Protein antibody
Anti-Nefm antibody
Anti-Nef3 antibody
Anti-Nfm antibody

Information sourced from Uniprot.org

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