• Immunofluorescence analysis of U-2 OS cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NDUFB4 in paraffin-embedded rat kidney using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NDUFB4 in paraffin-embedded mouse kidney using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NDUFB4 in paraffin-embedded human liver using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and NDUFB4 knockout (KO) HeLa cells, using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFB4 antibody (1-90) (STJ115762)

SKU:
STJ115762

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFB4 (1-90) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB4
Gene ID: 4710
Uniprot ID: NDUB4_HUMAN
Immunogen Region: 1-90
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
Immunogen Sequence: MSFPKYKPSSLRTLPETLDP AEYNISPETRRAQAERLAIR AQLKREYLLQYNDPNRRGLI ENPALLRWAYARTINVYPNF RPTPKNSLMG
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 4
Complex I-B15
Ci-B15
Nadh-Ubiquinone Oxidoreductase B15 Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 4 antibody
Anti-Complex I-B15 antibody
Anti-Ci-B15 antibody
Anti-Nadh-Ubiquinone Oxidoreductase B15 Subunit antibody
Anti-NDUFB4 antibody

Information sourced from Uniprot.org

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