![Immunofluorescence analysis of U-2 OS cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94772/376557/STJ115762_1__85679.1713143649.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of L929 cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94772/376558/STJ115762_2__24554.1713143649.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of NDUFB4 in paraffin-embedded rat kidney using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94772/376559/STJ115762_3__92493.1713143650.jpg?c=1 "Immunohistochemistry analysis of NDUFB4 in paraffin-embedded rat kidney using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of NDUFB4 in paraffin-embedded mouse kidney using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94772/376560/STJ115762_4__20328.1713143651.jpg?c=1 "Immunohistochemistry analysis of NDUFB4 in paraffin-embedded mouse kidney using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of NDUFB4 in paraffin-embedded human liver using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94772/376561/STJ115762_5__83647.1713143651.jpg?c=1 "Immunohistochemistry analysis of NDUFB4 in paraffin-embedded human liver using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and NDUFB4 knockout (KO) HeLa cells, using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94772/376562/STJ115762_6__79505.1713143652.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NDUFB4 knockout (KO) HeLa cells, using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of various lysates using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94772/376563/STJ115762_7__90592.1713143653.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/94772/376557/STJ115762_1__85679.1713143649.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] NDUFB4 Rabbit polyclonal antibody (STJ115762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-NDUFB4 antibody (1-90) (STJ115762)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NDUFB4 (1-90) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NDUFB4 |
| Gene ID | 4710 |
| Uniprot ID | NDUB4_HUMAN |
| Immunogen Region | 1-90 |
| Immunogen Sequence | MSFPKYKPSSLRTLPETLDP AEYNISPETRRAQAERLAIR AQLKREYLLQYNDPNRRGLI ENPALLRWAYARTINVYPNF RPTPKNSLMG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1). |
Additional Info
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Protein Name | Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 4Complex I-B15Ci-B15Nadh-Ubiquinone Oxidoreductase B15 Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembraneSingle-Pass Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 4 antibodyAnti-Complex I-B15 antibodyAnti-Ci-B15 antibodyAnti-Nadh-Ubiquinone Oxidoreductase B15 Subunit antibodyAnti-NDUFB4 antibody |
Information sourced from Uniprot.org